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Key Documents

PLA0269

Sigma-Aldrich

Rabbit anti-HSP60 Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

Synonym(s):

60 kDa chaperonin, CPN60, GroEL, HLD4, HSP-60, HSP60, HSP65, Heat shock protein 60, HuCHA60, P60 lymphocyte protein, SPG13, chaperonin 60, heat shock 60kD protein 1 (chaperonin), heat shock 60kDa protein 1 (chaperonin), heat shock protein 65, mitochondrial matrix protein P1, short heat shock protein 60 Hsp60s1, spastic paraplegia 13 (autosomal dominant)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

grade

Powered by Bethyl Laboratories, Inc.

species reactivity

human, mouse

technique(s)

immunohistochemistry: 1:200- 1:1,000
immunoprecipitation (IP): 2-5 μg/mg
western blot: 1:2,000- 1:10,000

accession no.

NP_002147.2

shipped in

wet ice

storage temp.

2-8°C

Gene Information

rabbit ... HSP60(3329)

General description

The HSPD1 (heat shock protein family D member 1) gene encodes the HSP60 (heat shock protein 60) subunit of the HSP60/HSP10 chaperonin complex. The HSPD1 gene consisting of 12 exons is localized to human chromosome 2q33.1.

Immunogen

The epitope recognized by PLA0269 maps to a region between residue 250 and 300 of human Heat Shock Protein 60 using the numbering given in entry NP_002147.2 (GeneID 3329).

Biochem/physiol Actions

The protein encoded by the HSPD1 (heat shock protein family D member 1) gene forms a double-barrel chaperonin complex with heat shock protein 10 (HSP10). This chaperonin complex facilitates folding of proteins to their native state in the mitochondrial matrix space. Missense mutation in the HSP60 gene has been associated with a rare dominant form of hereditary spastic paraplegia and a recessively inherited white matter disorder called MitCHAP60 disease.
HSP60 is also found to interact with the transcription factor interferon regulatory factor 3 (IRF3), which is involved in the induction of IFN-β (interferon- β) signaling pathway, a crucial host-protective response.

Physical form

Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide

Other Notes

Heat shock protein 60 (HSP60) is a member of the chaperonin family. HSP60 is a mitochondrial protein that may function as a signaling molecule in the innate immune system. It is essential for the folding and assembly of newly imported proteins in the mitochondria. [taken from NCBI Entrez Gene (GeneID: 3329)].

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Disease-associated mutations in the HSPD1 gene encoding the large subunit of the mitochondrial HSP60/HSP10 chaperonin complex.
Bross P and Paula F G
Frontiers in molecular biosciences, 3, 49-49 (2016)
Xiao-shan Li et al.
PloS one, 9(9), e107507-e107507 (2014-09-11)
Heat shock protein 60 (HSP60) is a chaperonin with essential functions for cell physiology and survival, and its expression correlates with prognosis in a number of malignancies. The aim of this study is to determine the relationship of HSP60 status
Lan Lin et al.
PloS one, 9(12), e114874-e114874 (2014-12-17)
The production of IFN- I (IFN-α/β) is one of the earliest and most important host-protective responses. Interferon regulatory factor 3 (IRF3) is a critical transcriptional factor in the IFN-β signaling pathway. Although significant progress has been achieved in the regulation
Sylvie Bannwarth et al.
Brain : a journal of neurology, 137(Pt 8), 2329-2345 (2014-06-18)
Mitochondrial DNA instability disorders are responsible for a large clinical spectrum, among which amyotrophic lateral sclerosis-like symptoms and frontotemporal dementia are extremely rare. We report a large family with a late-onset phenotype including motor neuron disease, cognitive decline resembling frontotemporal

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