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P3164

Sigma-Aldrich

Anti-Pig Serum antibody produced in rabbit

whole antiserum

Synonym(s):

Anti-Serum, pig

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

pig

technique(s)

immunoelectrophoresis: suitable

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Guinea pig serum is highly enriched with L-asparaginase activity. This L-asparaginase activity imparts inhibitory or anti-lymphoma effects to pig serum.

Specificity

Strong reactivity with normal pig serum has been determined.

Application

Anti-Pig Serum antibody produced in rabbit has been used in differential staining.
Anti-Pig Serum antibody produced in rabbit has been used in the dual differential staining of blastocysts to assess the number of inner cell mass (ICM) cells and trophectoderm (TE) cells.

Quality

The antiserum has been treated to remove lipoproteins.

Physical form

Rabbit Anti-Pig Serum is provided as a liquid containing 0.1% sodium azide as preservative.

Storage and Stability

For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Koji Yoshioka et al.
Biology of reproduction, 66(1), 112-119 (2001-12-26)
We evaluated the in vitro development of porcine zygotes that were cultured in a novel culture medium, porcine zygote medium (PZM), under different conditions and compared to in vivo development. The viability of these zygotes to full term after culture
Z Macháty et al.
Biology of reproduction, 59(2), 451-455 (1998-08-01)
In vitro development of early porcine embryos under different culture conditions was evaluated and compared to in vivo development. First, one- and two-cell embryos were collected and cultured individually in 20- microl drops under 5% CO2 in air for 4
Evidence that the L-Asparaginase Activity of Guinea Pig Serum is responsible for its Antilymphoma Effects
Broome JD
Nature, 191, 1114-1115 (1961)
Z Macháty et al.
Biology of reproduction, 57(5), 1123-1127 (1997-11-22)
Thimerosal (200 microM) triggered Ca2+ oscillations in 56 of 56 mature porcine oocytes. The Ca2+ oscillations were blocked by the sulfhydryl-reducing agent dithiothreitol (DTT), thus supporting the hypothesis that thimerosal acts by oxidizing critical sulfhydryl groups on intracellular Ca2+-release proteins.
Tao Lin et al.
Reproductive toxicology (Elmsford, N.Y.), 75, 96-109 (2017-12-17)
In this study, we used a pig model to investigate the effects of α-solanine (a natural toxin found mainly in potato sprouts) on oocyte maturation, quality and subsequent embryonic development. We found that α-solanine (10 μM) disturbed meiotic resumption and increased

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