P3076

Platelet-Derived Growth Factor-AA human

PDGF-AA, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): PDGF−AA
• MDL number: MFCD00081862
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE and HPLC)
• form: lyophilized powder
• potency: 1.0-3.0 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: 28.5 kDa (containing 250 amino acid residues)
• packaging: pkg of 10 μg; pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: <1 EU/μg
• UniProt accession no.: P04085
• storage temp.: −20°C
• Gene Information: human ... PDGFRA(5154)

Description

General description

Platelet-derived growth factor (PDGF) is composed two polypeptide subunits of A and B chain. PDGF is produced by a number of cells including smooth muscle cells, endothelial cells and macrophages. The PDGF gene is highly conserved and is mapped to human chromosome 7p22.3. PDGF is structurally and functionally related to vascular endothelial growth factors.

Biochem/physiol Actions

Cell division and relocalization of pulmonary artery smooth muscle cells and fibroblasts is known to be triggered by platelet-derived growth factor (PDGF). Due to its proliferatory function, it is associated with fibroproliferative disorders such as atherosclerosis, pulmonary arterial hypertension and lung fibrosis. PDGF exists both in homo and heterodimeric forms and stimulates α and β-cell surface receptors. Vascular endothelial growth factor expression is regulated by PDGF-AA autocrine signalling and is known to promote tumor angiogenesis in vivo.

Other Notes

Homodimer of the A chain of human PDGF.

Physical form

Sterile-filtered through a 0.2 μm filter. Lyophilized with 10 mM acetic acid.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable