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P0389

Sigma-Aldrich

Penicillinase from Bacillus cereus

lyophilized powder, 1,500-3,000 units/mg protein (using benzylpenicillin)

Synonym(s):

β-Lactamase I from Bacillus cereus, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase

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About This Item

CAS Number:
9073-60-3
Enzyme Commission number:
3.5.2.6 (BRENDA, IUBMB)
MDL number:
MFCD00131819
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Bacillus sp. (Bacillus cereus)

Quality Level

300

form

lyophilized powder

specific activity

1,500-3,000 units/mg protein (using benzylpenicillin)

composition

Protein, ~10%

technique(s)

activity assay: suitable

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

2-8°C

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General description

Penicillinase from Bacillus cereus has been shown to contain three distinct species when purified and examined for homogeneity by sedimentation analysis, amino-terminal analysis, and vertical acrylamide gel electrophoresis.

Application

Penicillinase can be used to reduce levels of penicillin in patients with severe penicillin reactions.
Used in the production of penicillin.
Used in the production of penicillin. Penicillinase from Bacillus cereus has been used in a comparison study with penicillinase from Bacillus subtillis where the Michaelis constant, shape of the Ph/activity curve and immunological properties were shown to be significantly different.

Biochem/physiol Actions

Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.

Unit Definition

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Physical form

Lyophilized powder containing phosphate and citrate buffer salts

Analysis Note

Protein determined by biuret.

inhibitor

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317 - H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cristian Piras et al.
Chemical science, 13(6), 1746-1758 (2022-03-15)
Large-scale population screening for early and accurate detection of disease is a key objective for future diagnostics. Ideally, diagnostic tests that achieve this goal are also cost-effective, fast and easily adaptable to new diseases with the potential of multiplexing. Mass
Properties of penicillinase from Bacillus cereus 569.
J Imsande et al.
The Journal of biological chemistry, 245(9), 2205-2212 (1970-05-10)
A Comparison of the Properties of Penicillinase produced by Bacillus subtilis and Bacillus cereus with and without Addition of Penicillin
Manson, E., et al.
Microbiology, 11, 493-505 (1954)
M A van Opstal et al.
Journal of pharmaceutical and biomedical analysis, 8(1), 49-60 (1990-01-01)
An automated assay for the determination of penicillin in formulations suitable for use in pharmaceutical quality control is presented. The method is based on the classical iodometric penicillin assay which is incorporated in a flow injection analysis (FIA) system. The
Junichiro Marui et al.
Journal of bioscience and bioengineering, 110(1), 8-11 (2010-06-15)
Aspergillus oryzae penicillin biosynthetic genes were clustered. The penicillin production was positively regulated by VeA, a global gene regulator required for transcriptional expression of the penicillin biosynthetic genes. Overexpression of the biosynthetic genes by a strong promoter yielded a greater
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