P0117

Monoclonal Anti-PIAS3 antibody produced in mouse

~2 mg/mL, clone PIA3, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Protein Inhibitor of Activated STAT 3
• MDL number: MFCD09265430
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: PIA3, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~68 kDa (additional band at 44 kDa may be present)
• species reactivity: chicken, mouse, canine, human, bovine
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 2-4 μg/mL using HepG2 total cell extract
• isotype: IgG1
• UniProt accession no.: Q9Y6X2
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PIAS3(10401) ; mouse ... Pias3(229615)

Description

General description

Protein inhibitor of activated STAT protein 3 (PIAS3) is an inhibitor of activated STAT3. The gene encodes for a 619-amino acid protein and has a molecular mass of 68 kDa. PIAS3 shares 55% identity with PIAS1 and PIASX proteins and 39% identity with PIASY. PIAS3 is widely expressed in various human tissues.

Immunogen

synthetic peptide corresponding to amino acids 591-604 of human PIAS3.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Monoclonal Anti-PIAS3 antibody produced in mouse has been used in:

• enzyme linked immuno sorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

Biochem/physiol Actions

Protein inhibitor of activated STAT protein 3 (PIAS3) and other members of this family can act as small ubiquitin-like modifier (SUMO) E3 ligases and interfere with the transcription activity of many transcription factors. PIAS3 binds to microphthalmia-associated transcription factor (MITF), a key DNA-binding protein. It blocks MITF DNA-binding activity and co-expression of MITF. PIAS3 in NIH 3T3 cells inhibits MITF-driven transcription activity.

Target description

PIAS3 encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves