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N6760

Sigma-Aldrich

Nutrient Mixture F-12 Ham

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Ham’s F-12

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: yes
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

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Application

Developed for growth of primary rat hepatocytes and rat prostate epithelial cells. Also used in a clonal toxicity assay using CHO cells.

Quantity

Formulated to contain 10.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.176 g/L sodium bicarbonate.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Muath Helal et al.
Frontiers in microbiology, 11, 147-147 (2020-03-03)
Invasive fungal infections (IFI) cause devastating morbidity and mortality, with the number of IFIs more than tripling since 1979. Our laboratories were the first to demonstrate that radiolabeled microorganism-specific monoclonal antibodies are highly effective for treatment of experimental fungal, bacterial
Susana P Mejía et al.
Frontiers in pharmacology, 12, 685391-685391 (2021-07-16)
Infectious diseases caused by intracellular microorganisms represent a significant challenge in medical care due to interactions among drugs during coinfections and the development of resistance in microorganisms, limiting existing therapies. This work reports on itraconazole (ITZ) encapsulated into functional polymeric
Claudia Maria Bertan Membrive et al.
Journal of animal science and biotechnology, 5(1), 25-25 (2014-05-30)
Estradiol (E2) is required for luteolysis in cows and its injection stimulates prostaglandin F2α (PGF2α) release. The main goal of our study was to investigate the ability of endometrial explants and cells treated with E2 and the calcium ionophore (CI)
Rani Ojha et al.
The Journal of urology, 191(6), 1889-1898 (2014-01-21)
We evaluated the status of autophagy in different grades of urothelial carcinoma and explored autophagy modulators as a potential adjunctive therapeutic agent for urothelial carcinoma. The study was performed in tumor tissue from patients with low and high grade urothelial
Ik-Jung Kim et al.
Cell host & microbe, 23(5), 583-593 (2018-05-11)
Helicobacter pylori (Hp) vacuolating cytotoxin (VacA) is a bacterial exotoxin that enters host cells and induces mitochondrial dysfunction. However, the extent to which VacA-dependent mitochondrial perturbations affect overall cellular metabolism is poorly understood. We report that VacA perturbations in mitochondria

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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