MAK474

Acetate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• UNSPSC Code: 12161503

Properties

• Quality Level: 100
• application(s): agriculture; environmental; food and beverages; pharmaceutical
• detection method: colorimetric; fluorometric
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Acetate is a common anion and fundamental to all forms of life. When bound to coenzyme A, it is central to the metabolism of carbohydrates and fats. Its acid form, acetic acid, is produced and excreted by acetic acid bacteria, such as Acetobacter genus and Clostridium acetobutylicum, which are found universally in food, water, and soil. Acetic acid is also a component of the vaginal lubrication of humans and other primates, where it appears to serve as a mild antibacterial agent. Acetic acid is the main component of vinegar, and is extensively used in food, dyes, paints, glue and synthetic fibers.

Features and Benefits

• Detection range: 0.20 to 20 mM for the colorimetric assay and 0.13 to 2 mM for the fluorometric assay.

Suitability

The kit is suitable for acetate activity determination in serum, plasma, food, agriculture and environmental samples, as well as for studying the effects of drugs on acetate metabolism.

Principle

The Acetate Assay Kit uses enzyme-coupled reactions to form a colored or fluorescent product. The color absorbance at 570 nm or fluorescence intensity at λEx  = 530 nm/λEm = 585 nm is directly proportional to theacetate concentration in the sample.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3