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Key Documents

MAK244

Sigma-Aldrich

Plasmin Activity Assay Kit (Fluorometric)

sufficient for 100 fluorometric tests

Synonym(s):

Plasmin Fluorometric Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

detection method

fluorometric

relevant disease(s)

cancer; hematological disorder

storage temp.

−20°C

General description

Plasmin (EC 3.4.21.7) is a serine protease occurring in plasma as plasminogen. Upon activation via cleavage by plasminogen activators; plasmin solubilizes fibrin clots and activates and/or degrades compounds of the coagulation and complement systems. Plasminogen-Plasmin system has also been implicated in a wide variety of physiologic and pathologic processes, including tumor growth, invasion and metastasis.

Suitability

Suitable to determine the activity of pure plasmin and to detect the activity of plasmin in plasma.

Principle

The Plasmin Activity Assay Kit is based on the ability of plasmin to proteolytically cleave a synthetic substrate and release a fluorophore, AMC, which can be easily quantified by fluorescence microplate readers. This assay kit is simple, rapid, and can detect plasmin activity as low as 10 ng in a variety of samples.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kwaan H C and McMahon B
Coagulation in Cancer, 43-66 (2009)
Goss N.
Production of Plasma Proteins for Therapeutic Use, 2012, 496 -496 null
Does plasmin have anticoagulant activity?.
Hoover-Plow J.
Vascular Health, 6, 199-199 (2010)

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