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M8662

Sigma-Aldrich

Mineral oil

PCR Reagent

Synonym(s):

Mineral oil for PCR

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About This Item

CAS Number:
8042-47-5
EC Number:
232-455-8
MDL number:
MFCD00131611
UNSPSC Code:
12181504
NACRES:
NA.52

grade

PCR Reagent

Quality Level

100

form

liquid

packaging

vial of 1.5 mL (Total volume 7.5 mL (5 vials))

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

storage temp.

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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General description

Mineral oil is used as a reaction mix overlay in polymerase chain reactions (PCR) to prevent reaction solutions from evaporating during the thermal cycling in PCR instruments without heated lids.

Application

Mineral oil has been used:
  • for routine PCR amplifications
  • to improve the heat conductivity between the device and the cycler during reverse transcription-polymerase chain reaction (RT–PCR) amplification
  • to prevent evaporation during single-cell MATQ-sequencing performed on Bravo automated liquid handling platform

Features and Benefits

  • Provided in a convenient 5 × 1.5 mL (1 vial) pack size
  • Tested for the absence of DNase, RNase, and protease.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hartmut Kaiser et al.
Plant physiology, 143(2), 1068-1077 (2006-12-13)
The response of stomata to a reduction of air humidity is composed of a hydropassive opening followed by active closure. Whereas the mechanisms behind the hydropassive opening are largely understood, the location and physiological basis of the sensing mechanisms leading
Ivan K Dimov et al.
Nature communications, 5, 3451-3451 (2014-03-29)
Discriminating cellular heterogeneity is important for understanding cellular physiology. However, it is limited by the technical difficulties of single-cell measurements. Here we develop a two-stage system to determine cellular heterogeneity. In the first stage, we perform multiplex single-cell RNA cytometry
Hsin-I Jen et al.
eLife, 8 (2019-04-30)
The mammalian cochlea loses its ability to regenerate new hair cells prior to the onset of hearing. In contrast, the adult vestibular system can produce new hair cells in response to damage, or by reprogramming of supporting cells with the
Neus Bota-Rabassedas et al.
Cell reports, 35(3), 109009-109009 (2021-04-22)
Cancer cells function as primary architects of the tumor microenvironment. However, the molecular features of cancer cells that govern stromal cell phenotypes remain unclear. Here, we show that cancer-associated fibroblast (CAF) heterogeneity is driven by lung adenocarcinoma (LUAD) cells at
Keith E Herold et al.
Methods in molecular biology (Clifton, N.J.), 504, 441-458 (2009-01-23)
A prototype handheld, compact, rapid thermocycler was developed for multiplex analysis of nucleic acids in an inexpensive, portable configuration. Instead of the commonly used Peltier heating/cooling element, electric thin-film resistive heater and a miniature fan enable rapid heating and cooling
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Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D4184)

Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D9307)

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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