G9768

Guanosine 5′-triphosphate–Agarose

aqueous glycerol suspension

• Synonym(s): 5′-GTP agarose
• MDL number: MFCD00171655
• UNSPSC Code: 41106500
• NACRES: NA.56

Properties

• biological source: bacterial (CORYNEBACTERIUM SP); enzyme from Porcine brain; plant (Sea weed); yeast
• Quality Level: 200
• form: aqueous glycerol suspension
• extent of labeling: 1-5 μmol per mL
• matrix: cross-linked 4% beaded agarose
• matrix activation: cyanogen bromide
• matrix attachment: ribose hydroxyls
• matrix spacer: 11 atoms (adipic acid dihydrazide)
• storage temp.: −20°C

Description

Application

Guanosine 5′-triphosphate-agarose (5′-GTP agarose) is an agarose conjugate in aqueous glycerol suspension used in affinity chromatography, for purification of guanosine-binding proteis

Physical form

Guanosine 5′-triphosphate–Agarose has been used:

• in chromatographic column for the purification of a translocation stimulating factor
• in GTP-Agarose pull down assays to measure the levels of GTP-bound RHEB (Ras homolog enriched in brain)
• in GTP-Binding assay
• for the depletion of GTP binding proteins in cytosol of rat liver

Suspension in 50% glycerol containing 0.25 M NaCl

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3