F1056
Human Fibronectin
from human plasma, powder, suitable for cell culture
Synonym(s):
Fibronectin
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About This Item
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product name
Fibronectin human plasma, lyophilized powder, BioReagent, suitable for cell culture, ≥95% (SDS-PAGE)
biological source
human plasma
Quality Level
sterility
sterile
product line
BioReagent
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
packaging
pkg of 1 mg
pkg of 2 mg
pkg of 5 mg
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑5 μg/cm2
solubility
H2O: 1 mg/mL at 37 °C
UniProt accession no.
Binding Specificity
Peptide Source: Collagen
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... FN1(2335)
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General description
Fibronectin is a class of high-molecular-weight adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The FN gene, which is highly conserved, gives rise to a minimum of 20 isoforms through alternative splicing in humans. These isoforms include notable variants such as cellular FN and plasma FN. Plasma FN is primarily synthesized in the liver, whereas cellular FN which represents only 5% of the circulating fibronectins is locally produced by various cell types.
Application
Fibronectin human plasma has been used:
- in neural selection medium to coat tissue culture surface for neuronal differentiation
- to coat culture vessels for characterization of iPSC and iPSC‑derived BMEC in 2D cell culture
- to coat culture supports for endothelial cell culture
Biochem/physiol Actions
Fibronectin plays a major role in biological processes. It interacts with surface receptors and other major ECM proteins like collagen to mediate cellular signals. Fibronectin is involved in a variety of cellular functions, including adhesion, cytoskeletal organization, oncogenic transformation, cell migration, phagocytosis, hemostasis, and embryonic differentiation. This glycoprotein can bind to integrins, collagens, fibrins and glycosaminoglycans (GAGs). FN plays a crucial role in promoting cell cycle progression and proliferation. This interaction influences cyclins and immediate early response genes, contributing to cellular processes related to cell division and growth. FN is also involved in cell survival and protection from apoptosis in various cell types, including epithelial and endothelial cells and leukocytes. FN serves as a critical factor in promoting cell survival and preventing programmed cell death in these cellular contexts. Mutations in the FN gene are associated with FN glomerulopathy and spondylometaphyseal dysplasia. Fibronectin plays a significant role in the wound healing process, and in extracellular matrix (ECM) assembly, hence is considered suitable for tissue engineering applications.
Caution
The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.
Preparation Note
This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.
In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.
In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Kunal Sharma et al.
eLife, 10 (2021-07-06)
Uropathogenic Escherichia coli (UPEC) proliferate within superficial bladder umbrella cells to form intracellular bacterial communities (IBCs) during early stages of urinary tract infections. However, the dynamic responses of IBCs to host stresses and antibiotic therapy are difficult to assess in
Jianfei He et al.
Redox biology, 36, 101631-101631 (2020-08-19)
Anastellin (AN), a fragment of the first type III module in fibronectin (FN), initiates formation of superfibronectin, a polymer which resembles the native cell-derived fibrillar FN found in the extracellular matrix of many tissues, but which displays remarkably different functional
Sean M Anderson et al.
Biomaterials, 30(27), 4618-4628 (2009-06-23)
Growth factors are a class of signaling proteins that direct cell fate through interaction with cell-surface receptors. Although a myriad of possible cell fates stems from a growth factor binding to its receptor, the signaling cascades that result in one
Xiao-Qiang Yan et al.
Molecules (Basel, Switzerland), 24(9) (2019-05-06)
Cyclooxygenase-2 (COX-2) as a rate-limiting metabolism enzyme of arachidonic acid has been found to be implicated in tumor occurrence, angiogenesis, metastasis as well as apoptosis inhibition, regarded as an attractive therapeutic target for cancer therapy. In our research, a series
Bram Piersma et al.
Cell and tissue research, 374(1), 165-175 (2018-05-05)
Mechanosensing of fibroblasts plays a key role in the development of fibrosis. So far, no effective treatments are available to treat this devastating disorder. Spectrins regulate cell morphology and are potential mechanosensors in a variety of non-erythroid cells, but little
Articles
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Protocols
Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.
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