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P3X63Ag8U.1

94120802, mouse blood, Lymphoblast

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About This Item

UNSPSC Code:
41106514

product name

P3X63Ag8U.1, 94120802

biological source

mouse blood

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse Myeloma

Cell Line Description

P3x63Ag8U.1 is a derivative of the P3x63Ag8 cell line (Sigma Catalogue number. 85011401) from the mouse myeloma MOPC21, which was derived from a female BALB/c mouse. This variant no longer synthesises gamma-1 chains but still produces kappa light chains which are not secreted.

Application

This cell line is useful for fusion to spleen cells to produce hybridomas or for hybridisation studies with other myelomas or lymphomas. Can also be used for immunoglobulin production.

Culture Medium

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures at 5-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Hybridoma Fusion Partners Cell Lines

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines at sigmaaldrich.com

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