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biological source
human skin (brain metastasis)
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human malignant melanoma
Cell Line Description
Derived from a brain metastasis of a 62 year old male with melanoma. The cells bleb during culture.
Application
Tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 11,12
D16S539: 12
D5S818: 10,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 16,17
CSF1PO: 11
D13S317: 11,12
D16S539: 12
D5S818: 10,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 16,17
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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