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product name
WIL2.NS.6TG, 93031001
biological source
human spleen
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian B lymphocyte
Cell Line Description
A non Ig-secreting, HAT sensitive, 6-thioguanine resistant, human B lymphocyte. Derived from the parent line WIL2.NS (Sigma Catalogue number. 90112121).
Application
Immortalising antigen-stimulated human B-lymphocytes by somatic hybridisation
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 5x1,000-5x10,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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