45.6.TG1.7

90110506

• UNSPSC Code: 41106514

Properties

• biological source: mouse blood
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast
• products: Immunoglobulin G (IgG2b) ( kappa)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse BALB/c myeloma

Cell Line Description

Derived from a female BALB/c myeloma tumour MPC-11. The parental cell line has mutated during growth in 6-thioguanine to establish the drug resistant line 45.6.TG1.7. This line is HPRT negative and produces mouse IgG2b kappa immunoglobulins.

Application

Fusion partner, immunoglobulin production

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 4-5x100,000 cells/ml; 5% CO₂; 37°C. Cells achieve a maximum cell density of 1-1.5x1,000,000 cells/ml.

Other Notes

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