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product name
BHK21C13-3P, 84100501
biological source
hamster kidney
growth mode
Adherent
karyotype
2n = 44, pseudodiploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Hamster Syrian kidney
Cell Line Description
BHK21C13-3P is a subclone of the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells grow as monolayers and have the same virus susceptibility as the parent line.
Application
Virus replication studies
Culture Medium
GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
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