Skip to Content
Merck
All Photos(2)

Documents

71768

Sigma-Aldrich

4-Nitrophenyl phosphate disodium salt hexahydrate

chromogenic, ≥99.0% (enzymatic), powder or crystals

Synonym(s):

p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
333338-18-4
Molecular Weight:
371.14
Beilstein:
5324661
EC Number:
224-246-5
MDL number:
MFCD00066288
UNSPSC Code:
12352204
PubChem Substance ID:
57652360
NACRES:
NA.83

product name

4-Nitrophenyl phosphate disodium salt hexahydrate, suitable for enzyme immunoassay, ≥99.0% (enzymatic)

Assay

≥99.0% (enzymatic)

form

powder or crystals

technique(s)

enzyme immunoassay: suitable

impurities

≤0.1% free nitrophenol

storage temp.

2-8°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-nitrophenyl phosphate disodium salt hexahydrate has been used:
  • as a substrate to measure the kinetic parameters of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) phosphatase activity
  • as substrate to assay acid phosphatase activity
  • as substrate to assay bound antibodies with anti-rabbit/human alkaline phosphatase conjugate

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens
Schweiger A, et al.
Infection, 40(2), 139-152 (2012)
The phytase from antarctic bacterial isolate, Pseudomonas sp. JPK1 as a potential tool for animal agriculture to reduce manure phosphorus excretion
Park I and Cho J
African Journal of Agricultural Research, 6(6), 1398-1406 (2011)
Targeting PTEN using small molecule inhibitors
Mak LH and Woscholski R
Methods, 77, 63-68 (2015)
Antibodies against 4-hydroxy-2-nonenal modified epitopes recognized chromatin and its oxidized forms: role of chromatin, oxidized forms of chromatin and 4-hydroxy-2-nonenal modified epitopes in the etiopathogenesis of SLE
Al-Shobaili HA, et al.
Disease Markers, 33(1), 19-34 (2012)
Dora Pinto et al.
Nature, 583(7815), 290-295 (2020-05-19)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a newly emerged coronavirus that is responsible for the current pandemic of coronavirus disease 2019 (COVID-19), which has resulted in more than 3.7 million infections and 260,000 deaths as of 6 May 20201,2. Vaccine and therapeutic
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service