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Merck

71196

Sigma-Aldrich

Sodium acetate solution

BioUltra, for molecular biology, ~3 M in H2O

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About This Item

Empirical Formula (Hill Notation):
C2H3NaO2
CAS Number:
Molecular Weight:
82.03
Beilstein:
3595639
MDL number:
UNSPSC Code:
12161700
eCl@ss:
39021906
PubChem Substance ID:
NACRES:
NA.21

Pricing and availability is not currently available.

grade

for molecular biology

Quality Level

product line

BioUltra

form

liquid

concentration

~3 M in H2O

technique(s)

precipitation assay: suitable

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

7.0-8.0 (25 °C, 3 M in H2O)

anion traces

chloride (Cl-): ≤10 mg/kg
phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤20 mg/kg

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤50 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

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This Item
I1764I1633I5500
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

-

form

powder

form

powder

shipped in

ambient

shipped in

-

shipped in

ambient

shipped in

ambient

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Gene Information

cow ... INS(280829)

Gene Information

cow ... INS(280829)

Gene Information

cow ... INS(280829)

Gene Information

cow ... INS(280829)

Application

Sodium acetate solution has been used:
  • in DNA extraction[1]
  • in reverse crosslinking and DNA purification[2]
  • to precipitate Gata6 (GATA binding protein 6) cDNA as a part of high-purity plasmid preparation[3]

Biochem/physiol Actions

Sodium acetate serves as a fixed base. It is useful to rectify acidosis among acidotic patients.[4] Orginally, sodium acetate is used as dialysate for hemodialysis, but is known to cause cardiovascular instability.[1]

Other Notes

Basic method of DNA precipitation with ethanol[5]

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Sodium acetate infusion in critically ill trauma patients for hyperchloremic acidosis
McCague A, et al.
Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, 19(1), 24-24 (2011)
An ultra-low-input native ChIP-seq protocol for genome-wide profiling of rare cell populations
Brind Amour J, et al.
Nature Communications, 6, 6033-6033 (2015)
Genome-wide mapping of DNase I hypersensitive sites in plants
Plant Functional Genomics, 71-89 (2015)
D.M. Wallace
Methods in Enzymology, 152, 42-42 (1987)
Derivation of extraembryonic endoderm stem (XEN) cells from mouse embryos and embryonic stem cells
Niakan KK, et al.
Nature Protocols, 8(6), 1028-1028 (2013)

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