C9386
Casein Peptone
Acid digest of casein from bovine milk, 8-12% amino, suitable for biotechnology and microbiology
Synonym(s):
Casein hydrolyzates, Hy-Case® SF, Peptone from casein, casein acid hydrolysate
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product name
Hy-Case® SF, Casein acid hydrolysate, from bovine milk
biological source
bovine milk
Quality Level
form
powder
nitrogen analysis
8-12% amino, ≥12.5% total
loss
≤11% loss on drying
pH
5.4-7.4
application(s)
food and beverages
microbiology
storage temp.
10-30°C
suitability
Streptococcus spp.
Streptomyces spp.
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General description
Hy-Case® SF is a high-quality acid hydrolysis of casein. Casein is a rich source of amino nitrogen so on treatment with a strong acid, it gets hydrolysed, producing free amino acids and several short peptide fragments. Special processing keeps inorganic ions to a minimum, so Hy-Case® SF is virtually a high concentration of free amino acids with a minimum of salt. It is a refined grade hydrolysate with high clarity in solution and is food grade and is recommended where a mixture of highly soluble free amino acids is required. It is especially recommended when inorganic ion levels need to be kept at a minimum.
Application
Casein hydrolysate Hy-Case® SF was used in DMCY medium, during an experimental study conducted to investigate the role of ssgA gene product in promoting the sporulation and cell division in Streptomyces griseus. It may also be used in the YH medium in studies related to Halobacterium denitrificans sp. which is characterised as being carbohydrate-utilizing, denitrifying, extremely halophilic bacteria.
Recommended for use as a microbiological nutrient in laboratory media, fermentations, and tissue culture applications requiring a mixture of highly soluble free amino acids.
Linkage
Similar to Hy-Case Amino, but contains a maximum of 2.0% sodium chloride
Reconstitution
Recommended concentration: 25 g/L
Legal Information
Hy-Case is a registered trademark of Kerry Group
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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S Kawamoto et al.
Microbiology (Reading, England), 143 ( Pt 4), 1077-1086 (1997-04-01)
The ssgA gene of Streptomyces griseus B2682, when present in high copy number, results in both suppression of sporulation and fragmented growth of mycelia. Western analysis with polyclonal antibodies against the gene product (SsgA) revealed a close correlation between SsgA
G A Tomlinson et al.
International journal of systematic bacteriology, 36(1), 66-70 (1986-01-01)
Halobacterium denitrificans was one of several carbohydrate-utilizing, denitrifying, extremely halophilic bacteria isolated by anaerobic enrichment in the presence of nitrate. Anaerobic growth took place only when nitrate (or nitrite) was present and was accompanied by the production of dinitrogen.
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