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A5431

Sigma-Aldrich

Noble agar

Synonym(s):

Agar, Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
50405901
NACRES:
NA.75

form

powder

Quality Level

application(s)

food and beverages

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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General description

Noble agar, also known as agar or agar-agar, is a gelling hydrocolloid extracted from sea weeds such as Gelidium and Gracilaria. It′s structure contains repeating units of D-galactose and 3,6-anhydro-L-galactose, with a few differences, in addition to a low ester sulphate residue. Agarose and agaropectin are the key components of agar. Noble agar is majorly used as both human and animal food supplement. In addition, it is also used in industries for the preparation of solid microbiological media.

Application

Noble agar has been used as a component of growth medium for High grade serous ovarian cancer (HGSOC) cell lines for soft agar colony assay. It has also been used as a media component to culture anaerobic bacteria, Bacteroides thetaitaomicron
Noble agar has been used to make agar gel plates that acts as a standard solid support material:
  • to culture tumor spheroids
  • in lysoplate assay method of Osserman & Lawlor to measure lysozyme concentration in albumen or plasma
  • as BG11 media component to culture Scenedesmus obtusiusculus strains
Originally designed for use in Noble′s cyanide citrate agar.

Preparation Note

Further purified from bacteriological agar with careful specific washings.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Agar and agarose biotechnological applications
Armisen R
Hydrobiologia, 157-166 (1991)
Agar
Food Gels, 1-51 (1990)
Effect of restricted preen-gland access on maternal self maintenance and reproductive investment in mallards
Giraudeau M, et al.
PLoS ONE, 5(10), e13555-e13555 (2010)
Mourad Sabri et al.
Infection and immunity, 77(3), 1155-1164 (2008-12-24)
Roles of the ZnuACB and ZupT transporters were assessed in Escherichia coli K-12 and uropathogenic E. coli (UPEC) CFT073. K-12 and CFT073 Deltaznu DeltazupT mutants demonstrated decreased (65)Zn(2+) uptake and growth in minimal medium. CFT073Deltaznu demonstrated an intermediate decrease of
Autophagy correlates with the therapeutic responsiveness of malignant pleural mesothelioma in 3D models
Barbone D, et al.
PLoS ONE, 10(8), e0134825-e0134825 (2015)

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