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A5306

Sigma-Aldrich

Bacteriological agar

for molecular biology

Synonym(s):

Agar, Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
9002-18-0
EC Number:
232-658-1
MDL number:
MFCD00081288
UNSPSC Code:
50405901
NACRES:
NA.31

grade

Molecular Biology

Quality Level

200

form

powder

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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General description

Bacteriological agar is a colloidal substance and a cell wall component, obtained from Rhodoyceae (marine algae) and Gelidium sp. Agarose combined with agaropectin forms this polysaccharide. It is also called as agar-agar and is a polymer of galactoside. This agar is soluble in boiling water but not in cold water.

Application

Bacteriological agar has been used:
  • as a component of ATCC agar for Escherichia coli culture
  • as one of the experimental diet feed for Atlantic salmon fry, to study the protein and lysine requirements for maintenance and for tissue accretion
  • as component of yeast extract peptone dextrose medium, Luria broth medium and synthetic complete drop out medium for Escherichia coli culture

Biochem/physiol Actions

Bacteriological agar is commonly used as a culture medium for microorganism. It is useful for fermentation process. Agar-agar serves as a preservative in food processing. It also possesses various other applications such as an emulsifier, carrier, lubricant, stabilizer, laxative disintegrant in pharmaceutical and cosmetic industries. Agar-agar is also used in photographic emulsion.

Quality

A purified agar in which the original naturally occurring pigments, salts and miscellaneous matter have been reduced to a minimum.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKCQ8213
MKCQ7151
MKCQ1339
MKCP2269
MKCN2927

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Natural Food Antimicrobial Systems, 417-417 (2000)
Handbook of Fillers, Extenders, and Diluents, 223-223 (2007)
Measuring the activity of protein variants on a large scale using deep mutational scanning
Fowler DM, et al.
Nature Protocols, 9(9), 2267-2267 (2014)
Protein and lysine requirements for maintenance and for tissue accretion in Atlantic salmon (Salmo salar) fry
Abboudi T, et al.
Aquaculture (Amsterdam, Netherlands), 261(1), 369-383 (2006)
Decontamination of materials contaminated with Francisella philomiragia or MS 2 bacteriophage using PES-Solid, a solid source of peracetic acid
Buhr TL, et al.
Journal of Applied Microbiology, 117(2), 397-404 (2014)
View All Related Papers

Protocols

Yeast Drop Out

Yeast Drop Out Bulletin. The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Although yeast can grow on a synthetic medium without any amino acids, better yield and growth rate can be achieved on richer media.

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