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70189

Millipore

Violet Red Bile Glucose Agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):

VRBG-Agar

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

Agency

according to ISO 21528-1:2017
according to ISO 21528-2:2017

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 13.0 g/L
bile salts, 1.5 g/L
crystal violet, 0.002 g/L
dextrose, 10.0 g/L
neutral red, 0.03 g/L
peptone (from gelatin), 7.0 g/L
sodium chloride, 5.0 g/L
yeast extract, 3.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

suitability

selective and differential for Citrobacter spp.
selective and differential for Enterococcus spp.
selective and differential for Escherichia coli
selective and differential for Klebsiella spp.
selective and differential for Proteus spp.
selective and differential for Salmonella spp.
selective and differential for Shigella spp.
selective and differential for coliforms
selective and differential for enterobacteriaceae

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Application

A selective medium for the isolation and enumeration of enterobacteria.

Preparation Note

Suspend 39.5g in 1 litre of distilled water and let soak. Bring to the boil to fully dissolve the powder. Sterilize by boiling for 1 minute with frequent agitation. DO NOT AUTOCLAVE. Prolonged heating in thermostatic bath could cause slight precipitates.

Other Notes

Enumeration of Enterobacteriaceae in milk

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD5570
BCCB9803
BCCB5488
BCBZ8614
BCBW8161

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D A Mossel et al.
Applied microbiology, 20(2), 273-275 (1970-08-01)
Samples of dried foods, feeds, and drugs were examined for Enterobacteriaceae by using buffered glucose-Brilliant Green-bile broth as the final enrichment medium and deep tubes of violet-red-bile-glucose-agar for confirmation. The pre-enrichment treatments used for the resuscitation of sublethally impaired cells
D.L. Cousins et al.
Journal of Food Protection, 53, 568-568 (1990)
D.A.A. Mossel et al.
The Journal of Applied Bacteriology, 26, 444-444 (1963)
Eleftheria Barouni et al.
Food chemistry, 200, 76-82 (2016-02-03)
The object of the current study was to develop a new process for continuous Feta-type cheese production using a biocatalyst consisting of immobilized rennin on a tubular cellulose/starch gel (TC/SG) composite, which has been proven to be an appropriate carrier

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