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Key Documents

15356

Sigma-Aldrich

Blotting-Nylon 66 membranes, type B, positive

for Northern and Southern blotting

Synonym(s):

Nylon 66-Blotting membranes, type B

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About This Item

UNSPSC Code:
41105339
NACRES:
NA.25

material

Nylon

Quality Level

100

grade

for molecular biology

form

roll

surface coverage

surface (quaternary ammonium groups) ≥99%

pore size

0.45 μm pore size

storage temp.

room temp

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General description

Blotting nylon provided on a roll is a standard item used in transfer of protein or nucleic acids during Western, Southern and Northern procedures. This membrane is positively charged nylon cast on a polyester support. It comes as a roll sized 0.2m x 3m, and can be cut to any size.

Application

Suitable for:
Protein transfer
Radioactive detection of blotted proteins and nucleic acids
Sensitive dot and slot blots of nucleic acids
for high-sensitive nucleic acid blotting, dot and lot blots. Particularly suitable for radioactive detection. Suitable for protein transfer and immunoblotting.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCBM6211V
BCBM6210V

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Gerardas Dambrauskas et al.
Journal of experimental botany, 54(383), 699-706 (2003-01-30)
Very little is known about the expression patterns in plants of genes that encode proteins involved in the initiation of DNA replication. Partial cDNA sequences that encode Cdc6 and Mcm3 in tobacco have been isolated. The sequences were used as
K Pfeifer et al.
Journal of cell science, 106 ( Pt 2), 545-553 (1993-10-01)
Ubiquitination of proteins is a critical step in the controlled degradation process of many polypeptides. Here we show that sponges, the simplest multicellular group of eukaryotic organisms, are also equipped with the ubiquitin pathway. The polyubiquitin cDNA was isolated and
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)

Protocols

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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