10378445001

Chromozym PK

90% (enzymatic), powder, pkg of 20 mg

• Synonym(s): C33H39N8O6COOCH3
• UNSPSC Code: 12352204

Properties

• description: Molecular Formula: C₃₃H₃₉N₈O₆COOCH₃
• Quality Level: 100
• Assay: 90% (enzymatic)
• form: powder
• mol wt: M_r 702.9
• packaging: pkg of 20 mg
• manufacturer/tradename: Roche
• storage temp.: room temp

Description

General description

Chromozym^® PK is a non-radioactive amidolytic chromogenic substrate mostly used in kinetic analysis of plasma kallikrein.

Application

Use Chromozym^® PK as a substrate for the determination of serine proteases, specifically plasma kallikrein in citrated plasma.

Quality

Contaminant: <0.5% free 4-nitraniline

Sequence

Benzoyl-prolyl-phenalanyl-arginine-4-nitranilide-acetate

Principle

Chromozym PK is cleaved by plasma kallikrein into a residual peptide and free 4-nitraniline, which is measured at 405 nm. The absorbance difference per minute is used for the determination of the kallikrein activity in U/ml.

Preparation Note

Activator: Mg²⁺ (K_m = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn²⁺, Fe²⁺, Zn²⁺)
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Chromozym is a registered trademark of Pentapharm AG, Basel

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable