MABS193

Anti-v-Src Antibody, clone 327

clone 327, from mouse

• Synonym(s): Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src, pp60c-src, p60-Src
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 327, monoclonal
• species reactivity: mouse
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_005408
• UniProt accession no.: P12931
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SRC(6714)

Description

General description

v-Src is the product of the Rous sarcoma virus (RSV) oncogene, and functions as a non-receptor tyrosine kinase belonging to the family of Src proteins. Srcs are typically activated by dimerized cell surface receptors such as integrins, receptor tyrosine kinases, and cytokine receptors. Activated Src kinases phosphorylate the cytoplasmic domains of these receptors enabling interaction between these receptors and their downstream effector molecules. Src proteins are involved in many cellular processs such as apoptosis, immunity, gene expression, cell adhesion, cell migration, and cell transformation. Previous studies have reported that v-Src plays an important role in tumorigenesis.

Immunogen

Recombinant protein corresponding to purified pp60src from bacterial recombinants which direct the synthesis of v-Src.

Application

Anti-v-Src Antibody, clone 327 detects level of v-Src & has been published & validated for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Immunological Signaling

Quality

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected v-Src in 10 µg of NIH/3T3 cell lysate.

Target description

~60 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
NIH/3T3 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable