FCMAB197F

Milli-Mark^® Anti-CD43-FITC Antibody, clone DF-T1

clone DF-T1, Milli-Mark^®, from mouse

• Synonym(s): Leukosialin, Leukocyte sialoglycoprotein, Sialophorin, Galactoglycoprotein, GALGP
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.44

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: FITC conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: DF-T1, monoclonal
• species reactivity: human
• manufacturer/tradename: Milli-Mark^®
• technique(s): flow cytometry: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001025459.1
• UniProt accession no.: P16150
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SPN(6693)

Description

General description

CD43 is a human heavily 0-glycosylated transmembrane protein, which is expressed by T cells and myeloid lineage cells (Borche, 1987; Stross, 1989). Reactivity with plasma cells has been observed (Beschomer, 1999). The molecule is known to be defective in the Wiskott-Aldrich syndrome and may participate in T-cell activation (Stross, 1989).
Immunoblotting studies have revealed molecular mass heterogeneity displayed by the antigen extracted from white blood cells of different lineage. Thus, two major bands of 140 and 130 kDa are seen for the promyelocytic cell line HL60, 110 kDa for the myeloblast cell line KG1 and a major 115 kDa band for peripheral blood lymphocytes (Stross, 1989).

Specificity

Antibody recognizes Human CD43 antigen.

Immunogen

Myeloblast cell line KG1

Application

Detect CD43 using this Milli-Mark Anti-CD43-FITC Antibody, clone DF-T1 validated for use in FC.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Quality

Evaluated by flow cytometry using human PBMCs

Target description

110 kDa (Stross, 1989)

Physical form

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Storage and Stability

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

Analysis Note

Control
PBMCs

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2