345860
Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10)
liquid, clone 2.2B10, Calbiochem®
Synonym(s):
Anti-GFAP
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
2.2B10, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
bovine, rat, mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GCM1(8521)
General description
Purified rat monoclonal antibody. Recognizes the ~55 kDa glial fibrillary acidic protein (GFAP).
Recognizes GFAP in astrocytes induced by a variety of CNS injuries in human and rat brain tissues.
This Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) is validated for use in ELISA, Immunoblotting, Frozen Sections, IP, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein.
Immunogen
Bovine Brain
bovine GFAP
Application
ELISA (0.1-0.5 µg/ml)
Immunoblotting (0.1-0.5 µg/ml)
Frozen Sections (10-50 µg/ml)
Immunoprecipitation (2-5 µg/ml)
Paraffin Sections (10-50 µg/ml, see comments)
Immunoblotting (0.1-0.5 µg/ml)
Frozen Sections (10-50 µg/ml)
Immunoprecipitation (2-5 µg/ml)
Paraffin Sections (10-50 µg/ml, see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Human or rat brain tissues
Human or rat brain tissues
Other Notes
Product is not to be used for animal treatment, in vivo research, or in any other contact procedure with livestock. Stains reactive rodent and human brain astrocytes induced by a variety of central nervous system injuries. This antibody is suitable for immunohistochemical staining of Bouin′s-fixed, frozen, or paraffin-embedded tissue sections. Variables associated with assay conditions will dictate the proper working dilution.
Tohyama, T., et al. 1993. Am. J. Pathol.142, 871.
Lee, V.M., et al. 1984. J. Neurochem. 42, 25.
Lee, V.M., et al. 1984. J. Neurochem. 42, 25.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Flora Guillot et al.
Journal of neuroinflammation, 12, 130-130 (2015-07-05)
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Primary and secondary traumatic brain injury (TBI) can cause tissue damage by inducing cell death pathways including apoptosis, necroptosis, and autophagy. However, similar pathways can also lead to senescence. Senescent cells secrete senescence-associated secretory phenotype proteins following persistent DNA damage
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