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1.52015

Supelco

Chromolith® Si

L × I.D. 100 mm x 10 mm column

Synonym(s):

Chromolith® SemiPrep Si 100-10 column

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About This Item

UNSPSC Code:
41115709

material

PEEK column

Quality Level

100

Agency

suitable for USP L3

product line

Chromolith®

feature

endcapped: no

packaging

1 ea of

parameter

150 bar pressure
45 °C max. temp.

technique(s)

HPLC: suitable (Semi Prep HPLC)
LC/MS: suitable

column L × I.D.

10 cm × 10 mm

matrix

monolithic silica

matrix active group

Silica bonding phase

pore size

13 nm mesoporosity
2 μm macroporosity

operating pH range

2-7.5

separation technique

hydrophilic interaction (HILIC)
normal phase

storage temp.

no temp limit

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General description

Chromolith® SemiPrep Si HPLC columns are ideally suited for direct scale-up from analytical to semiprep. This is because they offer faster sample throughput at a lower operating pressure compared to semi-prep columns packed with 5 μm particles.
Chromolith® SemiPrep 10 mm i.d. columns combine high separation speed with excellent performance. This makes them the perfect alternative to particulate columns of 10 mm i.d. (and even 21.2 mm i.d.). They have the same bimodal porous silica rod structure as Chromolith® analytical columns with an internal diameter of 4.6 mm. Their macropores are 2 μm in diameter and the mesopores are 13 nm. This combination dramatically reduces separation time while increasing efficiency.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes

The recommended Guard-columns are:
Chromolith® SemiPrep Si 10-10 guard cartridges (3 pieces) [1.52035] with the Chromolith® Guard Cartridge Holder 10 mm x 10 mm [1.52037]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Analysis Note

Pressure: ≤ 25 bar
Performance (2-nitroanisol)
Theoretical Plates (N/m) (2-nitroanisol): ≥ 80000
Capacity factor (2-nitroanisol): 1.4 - 1.9
Tailing (USP): 0.90 - 1.40 %
Selectivity
separation factor (2-nitroanisol/nitrobenzene): 3.45 - 3.95

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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