126579
Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free
Synonym(s):
Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free
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About This Item
CAS Number:
UNSPSC Code:
12352202
Assay
≥95% (Albumin, electrophoresis)
form
lyophilized powder
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤0.2% fatty acids
≤2.0% Ash
≤5% moisture
≤50 EU/mg endotoxins
color
pale green
solubility
aqueous buffer: 20 mg/mL
water: 20 mg/mL
shipped in
ambient
storage temp.
2-8°C
Related Categories
General description
Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free is designed for use in cell culture systems and animal perfusion studies. It has a pH of 7.0 (1% solution).
Bovine serum albumin (BSA), a member of the serum albumin family, contains three domains and two sub-domains under each. It is a non-glycosylated globular, α-helical protein that has 17-disulfide bonds.
Application
Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free has been used in the preparation of advanced glycation end-products (AGEs). It has also been used to dilute lipopolysaccharide (LPS) and tumor necrosis factor (TNF) and in the stimulation and fixation of whole blood cells.
Biochem/physiol Actions
Bovine Serum Albumin (BSA) is the most important constituent of cell culture media. It aids embryonic stem cells (hESC) differentiation. BSA facilitates the transport of drugs, hormones, and fatty acids. It elicits high solubility and is suitable in the cosmetic and pharmaceutical industries. It is applicable as a blocking agent in enzyme-linked immunosorbent assay (ELISA).
Warning
Toxicity: Standard Handling (A)
Preparation Note
Material may take up to 24 h to fully dissolve.
Reconstitution
Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ravi Kumar Murumalla et al.
Lipids in health and disease, 11, 175-175 (2012-12-25)
On the basis that high fat diet induces inflammation in adipose tissue, we wanted to test the effect of dietary saturated and polysunsaturated fatty acids on human adipose tissue and adipocytes inflammation. Moreover we wanted to determine if TLR2 and
Dan Ye et al.
Structure (London, England : 1993), 26(11), 1513-1521 (2018-09-18)
We introduce resonant soft X-ray scattering (RSoXS) as an approach to study the structure of proteins and other biological molecules in solution. Scattering contrast calculations suggest that RSoXS has comparable or even higher sensitivity than hard X-ray scattering because of
Kathryn J Newhall et al.
Clinical chemistry, 56(9), 1483-1491 (2010-07-29)
Biomarker assays are often conducted on whole blood samples in the course of drug development studies. Because bacterial lipopolysaccharide (LPS) (endotoxin) contamination is known to cause spontaneous cytokine production by monocytes, contamination of blood collection tubes may interfere with biomarker
Tamara Topală et al.
Clujul medical (1957), 87(4), 215-219 (2014-01-01)
The continuous search for new molecules with therapeutic abilities has led to the synthesis and characterization of a large number of metal complexes, proven to exhibit potential as pharmacological agents through their antibacterial, antiviral, antifungal and antineoplastic properties. As serum
Yuhong Xiao et al.
Journal of immunological methods, 384(1-2), 148-151 (2012-06-27)
The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter
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