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Key Documents

1.19612

Supelco

Superspher® 60 RP-8 (4 µm) HPLC Columns

HPLC sorbent

Synonym(s):

TM=["Superspher"] C8 sorbent for HPLC

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

Superspher® 60 RP-8,

material

stainless steel column

Agency

suitable for USP L7

product line

Superspher®

feature

endcapped: no

parameter

60 °C max. temp.

technique(s)

HPLC: suitable
mass spectrometry (MS): suitable (Low - Medium)

matrix

fully porous particle

matrix active group

C8 bonding phase

particle size

4 μm

pore size

60 Å pore size

operating pH range

2-7.5

bulk density

200‑400 kg/m3

separation technique

reversed phase

storage temp.

no temp limit

Related Categories

Application


  • An optimized procedure for the separation of amino acid phenylthiohydantoins by reversed-phase HPLC: This study demonstrates the use of Superspher 60 RP-8 for the high-resolution separation of amino acid derivatives, crucial in biochemical analysis of protein structures. Optimized HPLC protocols improve analytical accuracy and efficiency, beneficial for fields such as analytical chemistry and pharmaceutical analysis (Persson B, Eaker D, 1990).

Analysis Note

Application test: passes test
Theoretical Plates (N/m) (Anthracene): ≥ 105000
stability testing: passes test
selectivity test: passes test
separation factor (Lysine/Digitoxigenine): 1.6 - 2.1
separation factor (Lysine/PHB-propylester): 1.1 - 1.4
separation factor (Naphthalene/Digitoxigenin, selectivity test): 4.5 - 6.5
capacity factor (naphthalene): 17.0 - 22.0
Particle Size (d10): 3.4 - 4.0 µm
Particle Size (d50): 4.6 - 5.3 µm
Particle Size (d90): 6.4 - 7.2 µm

Legal Information

Superspher is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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