1.11350

Bactident^™ Kovac′s Indole reagent

Available in a practical dropper bottle, for Escherichia coli

• Synonym(s): 4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac, Indole reagent, Indole test reagent
• UNSPSC Code: 41106200
• NACRES: NA.84

Properties

• Quality Level: 100
• description: Bactident™ E. coli 1.13303
• form: liquid (ready-to-use)
• shelf life: 210 days
• feature: ready-to-use
• composition: (n-Butanol; Hydrochloric acid; 4 dimethylaminobenzaldehyde)
• packaging: pkg of (1 x 30 ml, Plastic bottle)
• technique(s): microbe id | specific enzyme detection: suitable
• pH: <1 (20 °C in H₂O)
• transition temp: flash point 36 °C
• density: 0.92 g/cm³ at 20 °C
• application(s): environmental; food and beverages
• storage temp.: 2-8°C
• suitability: Enterobacter spp.; Escherichia coli

Description

General description

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

Application

Bactident^™ Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

Linkage

Replaces: 1.11350.0007

Analysis Note

Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryptophan broth.

Legal Information

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS02,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H226 - H290 - H302 - H314 - H317 - H335 - H336
• Precautionary Statements: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): 96.8 °F
• Flash Point(C): 36 °C