04-151

Anti-IFNAR-1 Antibody, clone MARI-5A3

clone MARI-5A3, from mouse

• Synonym(s): interferon (alpha, beta and omega) receptor 1, beta-type antiviral protein, alpha-type antiviral protein, Type I interferon receptor 1, Cytokine receptor family 2 member 1, interferon-beta receptor 1, Cytokine receptor class-II member 1, IFN-alpha-REC, i
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: MARI-5A3, monoclonal
• species reactivity: mouse
• technique(s): flow cytometry: suitable; immunoprecipitation (IP): suitable; neutralization: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_034638
• UniProt accession no.: P33896
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Ifnar1(15975)

Description

General description

Interferon alpha/beta receptor 1 (IFNAR-1) is a single-pass type I membrane protein containing three fibronectin type-III domains and is a member of the type II cytokine receptor family. IFNAR-1 serves as an interferon receptor specific to interferons alpha and beta, a family of type I interferons critical to the promotion and the linking of adaptive and innate immune response. Type I interferons all bind to a heterodimeric receptor composed of two subunits, IFNAR-1 and IFNAR-2. The binding of the type I IFNs initializes tyrosine phosphorylation for several proteins such as; IFNR alpha/beta subunits, JAKs, STAT, and TYK2 proteins.

Specificity

This antibody recognizes the extracellular domain of IFNAR-1.

Immunogen

Recombinant protein corresponding to the extracellular domain of mouse IFNAR-1.

Application

Anti-IFNAR-1 Antibody, clone MARI-5A3 is an antibody against IFNAR-1 for use in FC, IP, WB, NEUT.

Western Blot Analysis: A previous lot was used by an independent laboratory in WB. (Sheehan, K., et al. (2006). Journal of Interferon & Cytokine Research. 26:804-819.)

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Sheehan, K., et al. (2006). Journal of Interferon & Cytokine Research. 26:804-819.)

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC. (Sheehan, K., et al. (2006). Journal of Interferon & Cytokine Research. 26:804-819.)

Neutralizing Assay: A previous lot was used by an independent laboratory in neutralization studies. (Sheehan, K., et al. (2006). Journal of Interferon & Cytokine Research. 26:804-819.)

Quality

Evaluated by Flow Cytometry in BALB/c splenocytes.

Flow Cytometry Analysis: 5 µg of this antibody detected IFNAR-1 in BALB/c splenocytes.

Target description

63 kDa calculated

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable