Skip to Content
Merck
  • Semiautomated laser capture microdissection of lung adenocarcinoma cytology samples.

Semiautomated laser capture microdissection of lung adenocarcinoma cytology samples.

Acta cytologica (2012-12-05)
Sinchita Roy Chowdhuri, Jeffrey Hanson, Jerome Cheng, Jaime Rodriguez-Canales, Patricia Fetsch, Ulysses Balis, Armando C Filie, Giuseppe Giaccone, Michael R Emmert-Buck, Jason D Hipp
ABSTRACT

In the past decade molecular diagnostics has changed the clinical management of lung adenocarcinoma patients. Molecular diagnostics, however, is largely dependent on the quantity and quality of the tumor DNA that is retrieved from the tissue or cytology samples. Frequently, patients are diagnosed on cytology specimens where the tumor cells are scattered within the cell block, making selecting for tumor enrichment difficult. In the past we have used laser capture microdissection (LCM) to select for pure populations of tumor cells to increase the sensitivity of molecular assays. This study explores several methods for semiautomated computer-guided LCM. Hematoxylin and eosin- or TTF-1-immunostained slides from a pleural effusion cell block with metastatic lung adenocarcinoma were used for LCM with either AutoScan or a recently described pattern-matching algorithm, spatially invariant vector quantization (SIVQ), to define morphologic predicates (vectors) to select cells of interest. We retrieved pure populations of tumor cells using both algorithm-guided LCM approaches with slight variations in cellular retrievals. Both methods were semiautomated, requiring minimum technical supervision. In this study we demonstrate the first semiautomated, computer-guided LCM of a cytology specimen using SIVQ and AutoScan, a first step towards the long-term goal of integrating LCM into the clinical cytology-molecular workflow.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Hematoxylin
Pricing and availability is not currently available.
Sigma-Aldrich
Hematoxylin, certified by the Biological Stain Commission
Pricing and availability is not currently available.