582709-U

BIOshell IgG 1000 Å C18, 2.7 μm HPLC Column

column L × I.D. 3 cm × 4.6 mm

• Synonym(s): BIOshell IgG 1000 Å C18, 2.7 μm HPLC Column
• UNSPSC Code: 41115700

Properties

• material: stainless steel column
• Agency: suitable for USP L1
• product line: BIOshell
• feature: endcapped
• packaging: 1 ea of
• extent of labeling: 1.4% Carbon loading
• technique(s): HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable
• column L × I.D.: 3 cm × 4.6 mm
• surface area: 22 m²/g
• matrix: Fused-Core^® particle platform; superficially porous particle
• matrix active group: C18 (diisobutyloctadecylsilane) bonding phase
• particle size: 2.7 μm
• pore size: 1000 Å pore size
• operating pH range: 1-8
• separation technique: reversed phase

Description

General description

BIOshell IgG 1000 Å C18 is a high-speed, high-performance liquid chromatography column based on a new wide-pore (1000 Å) Fused-Core^® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 μm thin porous shell and the small overall particle size of 2.7 μm. The sterically protected C18 bonded phase of BIOshell IgG 1000 Å C18 provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds, such as proteins.

Application

UHPLC Analysis of Reduced IgG1 on BIOshell IgG 1000 Å C18
UHPLC Analysis of Proteins on BIOshell IgG 1000 Å C18, 2.7 μm
UHPLC Analysis of Proteins on BIOshell IgG 1000 Å C18, 2.7 μm
UHPLC Analysis of IgG2 on BIOshell IgG 1000 Å C4, C18, and Diphenyl, 2.7 μm

Analysis Note

The target analytes include monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with molecular weights up to 500 kDa.

Legal Information

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.