567507-U

Discovery^® HS F5 (3 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

• Synonym(s): Discovery Pentafluorophenyl PFP HPLC Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• Product Name: Discovery^® HS F5 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L43
• product line: Discovery^®
• feature: endcapped
• manufacturer/tradename: Discovery^®
• packaging: 1 ea of
• extent of labeling: 12% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 15 cm × 4.6 mm
• surface area: 300 m²/g
• surface coverage: 4 μmol/m²
• impurities: <10 ppm metals
• matrix: silica gel, high purity, spherical particle platform; fully porous particle
• matrix active group: PFP (pentafluorophenyl) phase
• particle size: 3 μm
• pore size: 120 Å
• operating pH range: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log P_o/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.

The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Application

• Nature of the main contaminant in the drug primaquine diphosphate: SFC and SFC-MS methods of analysis.: This study uses the Discovery^® HS F5 HPLC Column to identify and quantify the main contaminant in primaquine diphosphate, crucial for ensuring the quality and safety of this antimalarial drug (Brondz et al., 2007).
• HPLC assisted chemobiological standardization of alpha-glucosidase-I enzyme inhibitory constituents from Piper longum Linn-An Indian medicinal plant.: Demonstrates the application of the Discovery^® HS F5 HPLC Column in the biologically relevant chemical analysis of Indian medicinal plants, contributing to the standardization of herbal medicine (Pullela et al., 2006).
• Disposition study of a new potential antineoplastic agent dimefluron in rats using high-performance liquid chromatography with ultraviolet and mass spectrometric detection.: Utilizes the Discovery^® HS F5 HPLC Column to study the pharmacokinetics and metabolism of a novel anticancer agent, aiding in the development of effective cancer therapies (Císar et al., 2005).
• A simplified analytical method for a phenotyping cocktail of major CYP450 biotransformation routes.: This research employs the Discovery^® HS F5 HPLC Column to simplify the analysis of a phenotyping cocktail, enhancing the assessment of drug metabolism and facilitating personalized medicine approaches (Jerdi et al., 2004).

Features and Benefits

• Unique selectivity
• Similar retention to C18 (sometimes requires stronger mobile phase)
• Excellent peak shape
• Stable, low-bleed LC-MS separations
• Scalable separations from 3 to 10μm particle sizes

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany