ZRB1700
Anti-β-Galactosidase Antibody, clone 1G21 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
EC:3.2.1.23; Beta-gal
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1G21, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 116.48 kDa
observed mol wt ~120 kDa
purified by
using Protein A
species reactivity
E. coli
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
N-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
bacterial ... LacZ(945006)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1G21 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects E. coli β-Galactosidase. It targets an epitope within 24 amino acids from the N-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of E.coli β-galactosidase.
Application
Quality Control Testing
Evaluated by Western Blotting with E. coli β-Galactosidase.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected E.coli β-Galactosidase.
Tested applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected β-Galactosidase in HeLa cells infected with Adenovirus expressing β-Galactosidase.
Enzyme Immunoassay Analysis (ELISA): A serial of dilutions from a representative lot detected E. coli β-Galactosidase.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with E. coli β-Galactosidase.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected E.coli β-Galactosidase.
Evaluated by Western Blotting with E. coli β-Galactosidase.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected E.coli β-Galactosidase.
Tested applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected β-Galactosidase in HeLa cells infected with Adenovirus expressing β-Galactosidase.
Enzyme Immunoassay Analysis (ELISA): A serial of dilutions from a representative lot detected E. coli β-Galactosidase.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with E. coli β-Galactosidase.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected E.coli β-Galactosidase.
Target description
Beta-galactosidase (UniProt: P00722; also known as EC:3.2.1.23; Beta-gal) is encoded by the LacZ gene (Gene ID: 945006) in E. coli. The β-galactosidase is a tetrameric enzyme composed of four polypeptide chains (A-D), each with 1023 amino acids. Each monomer contains five domains (1-5). Domain 3 has an /β or TIM barrel structure with the active site located at the C-terminal end of the barrel. The residues that form the active site are contributed by different segments of the polypeptide chain. β-galactosidase hydrolyzes the disaccharide lactose to generate galactose and glucose and can also convert lactose to allolactose, which is the natural inducer for the lac operon. It displays strict specificity at the galactosyl position but can hydrolyze β-D-galactopyranosides with a wide variety of aglycons. Its substrate binding site is localized to amino acids 538-571. The hydrolysis reaction is a double displacement reaction where the product retains the same stereochemistry as the starting state. In the initial step, the substrate forms a covalent -D-galactosyl enzyme intermediate with the nucleophile Glu537. In the second step the intermediate is released, a step that is facilitated by Glu461, which abstracts a proton from the acceptor molecule, R OH. The activity of β-galactosidase is dependent upon the presence of divalent (Mg2+) and monovalent (Na+) cations. Mg2+ causes a 5-to-100-fold activation, depending on the substrate. However, Mg2+ can be replaced by Mn2+ to obtain full activity. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mathews, BW. (2005). CR Biologics. 328(6); 549-556; Juers, DH., et al. (2001). Biochemistry. 40(49); 14781-14794).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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