ZMS1165
Anti-CHRM4 Antibody, clone 18C7.2 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Anti-ZFP36L2 Antibody, clone 1E10 ZooMAb® Rabbit Monoclonal, Muscarinic M4 receptor, Muscarinic acetylcholine receptor M4
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
mouse
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
18C7.2, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 53.05 kDa
observed mol wt ~56 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
ELISA: suitable
affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
Cytoplasmic domain
Protein ID accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... CHRM4(1132)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 18C7.2 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects Muscarinic acetylcholine receptor M4 (CHRM4). It targets an epitope within the third intracellular loop.
Immunogen
GST-tagged recombinant fragment corresponding to the third intracellular loop of human muscarinic acetylcholine receptor M4 (CHRM4).
Application
Quality Control Testing
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Muscarinic acetylcholine receptor M4 (CHRM4) in SHSY5Y cell lysate.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound full-length recombinant Human CHRM4 protein with a KD of 5.1 x 10-6 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CHRM4 in SH-SY5Y cells.
Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected full-length recombinant Human CHRM4.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Muscarinic acetylcholine receptor M4 (CHRM4) in SHSY5Y cell lysate.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound full-length recombinant Human CHRM4 protein with a KD of 5.1 x 10-6 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CHRM4 in SH-SY5Y cells.
Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected full-length recombinant Human CHRM4.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Muscarinic acetylcholine receptor M4 (UniProt: also known as Muscarinic M4 receptor) is encoded by the CHRM4 gene (Gene ID: 1132) in human. Acetylcholine receptors are classified as muscarinic or nicotinic. Muscarinic receptors are found in neuroeffector junctions and are coupled to G-proteins. On the other hand, nicotinic receptors are found in the autonomic ganglia and neuromuscular junctions. Muscarinic receptors are G-protein-coupled receptors that are involved in the transduction of cholinergic signals in the central nervous system, autonomic ganglia, smooth muscles, and other parasympathetic end organs. Five different subtypes of muscarinic receptors have been described (M1-M5). The M1, M3, and M5 receptors are excitatory receptors, whereas M2 and M4 receptors inhibit neuronal firing by decreasing the intracellular concentration of cAMP and activating G-protein coupled inward rectifying potassium channels. M1, M3, and M5 receptors are coupled to Gq/11 protein, while M2 and M4 pre
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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