T3160

TC-100 Insect Medium

With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture

• Synonym(s): BML-TC/10
• MDL number: MFCD00243217
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | insect: suitable
• components: glucose: 1.0 g/L; L-glutamine: 0.6 g/L; NaHCO₃: 0.35 g/L
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

TC-100 Insect Medium has been developed to optimize the production of Autographica californica nuclear polyhedrosis virus (NPV) virions by cells from the fall armyworm, Spodoptera frugiperda. It has been found to support the growth of cells derived from a variety of lepidopteran species. The medium is mostly employed in the growth of baculoviruses in lepidopteran cell lines.

Application

For baculovirus culturing in lepidoptera.

TC-100 Insect Medium has been used:

• in the transfection of insect cells in suspension for efficient baculovirus generation
• to harvest Varroa destructor saliva
• as a starter media to culture and maintain insect cells such as Spodoptera frugiperda

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable