SAB5900201

Alpaca Anti-GFP, recombinant single domain Antibody

Recombinant Alpaca(VHH) Nano antibody

• UNSPSC Code: 12352203

Properties

• biological source: alpaca
• Quality Level: 100
• recombinant: expressed in (E. coli)
• conjugate: unconjugated
• antibody form: (Affinity purified VHH antibody)
• clone: GE10, monoclonal
• Assay: >95% (SDS-PAGE)
• form: lyophilized solid
• mol wt: 13.7 kDa; ~15-18 kDa (SDS PAGE under reducing condition)
• purified by: (Affinity chromatography purified via Ni-charged resin); affinity chromatography (purified via Ni-charged resin)
• concentration: 1 mg/mL
• technique(s): ELISA: 1:2000-1:10000; immunofluorescence: 1:100-1:600; immunoprecipitation (IP): 0.5-4.0 μg; western blot: 1:1000-1:5000
• color: colorless
• pH: ( in PBS, pH 7.4)
• isotype: VHH
• Protein ID accession no.: BAG71039.1
• UniProt accession no.: A0A059PIQ0
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr (previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488 nm

Specificity

Green fluorescence protein (GFP) and other derivatives of GFP

Immunogen

Recombinant full length GFP protein

Application

ELISA, WB, IP, IF

Packaging

50 µg per vial / 100 µg per vial

Physical form

Purified VHH protein in buffer containing 25 mM TAPS pH8.5, 500 mM NaCl, 5 mM EDTA

Reconstitution

Reconstitute the lyophilized protein with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Storage and Stability

Stable for 1 years at 4°C from date of receipt.

Other Notes

Storage at 4°C; Do not freeze.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable