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SAB2702218

Sigma-Aldrich

Monoclonal Anti-6X His tag antibody produced in mouse

clone GT359, affinity isolated antibody

Synonym(s):

Mouse Anti-Hexa Histidine tag

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

GT359, monoclonal

form

buffered aqueous solution

species reactivity

human, mouse

concentration

1mg/mL

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: 500-3000

isotype

IgG3

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

6X His is a histidine tag that consists of a series of histidine residues at the amino or carboxy terminus of a protein.

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Application

Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, ICC/IF: 1:100-1:2000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Monoclonal Anti-6X His tag antibody produced in mouse has been used in Co-Immunoprecipitation (Co-IP) and immunoblotting.

Biochem/physiol Actions

6X His tag does not affect the structure and function of protein and can be used in antibody production. It is also used to purify proteins under native or denaturing form.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Purification: Affinity purified by Protein G

Physical form

Phosphate-buffered saline, no preservative added.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Disrupting CD147-RAP2 interaction abrogates erythrocyte invasion by Plasmodium falciparum.
Z Meng-Yao, et al.
Blood, 131(10), 1111-1121 (2018)
SKP2-and OTUD1-regulated non-proteolytic ubiquitination of YAP promotes YAP nuclear localization and activity.
Yao F, et al.
Nature Communications, 9(1), 2269-2269 (2018)
Jordan Demone et al.
PloS one, 17(12), e0277668-e0277668 (2022-12-15)
Agroinfiltration is a method used in biopharming to support plant-based biosynthesis of therapeutic proteins such as antibodies and viral antigens involved in vaccines. Major advantages of generating proteins in plants is the low cost, massive scalability and the rapid yield
Molecular Biology of Streptococci (2007)
Meng-Yao Zhang et al.
Blood, 131(10), 1111-1121 (2018-01-21)
Effective vaccines against malaria caused by Plasmodium falciparum are still lacking, and the molecular mechanism of the host-parasite interaction is not fully understood. Here we demonstrate that the interaction of RAP2, a parasite-secreted rhoptry protein that functions in the parasitophorous
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