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RAB0059

Sigma-Aldrich

Human MIP-3 α / CCL20 ELISA Kit

for serum, plasma, cell culture supernatant and urine

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type urine
sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1.5 pg/mL
standard curve range: 0.8-600 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CCL20(6364)

General description

The Human MIP-3 α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MIP-3 α in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human MIP-3 α

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

C-C motif chemokine ligand 20 (CCL20), also known as macrophage-inflammatory protein-3 α, recruits inflammatory and epithelial cells. It acts as a ligand for C-C chemokine receptor-6 (CCR6). The protein has a role in the growth and metastasis of pancreatic cancer.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Production of CCL20 from lung cancer cells induces the cell migration and proliferation through PI3K pathway
Beibei Wang
Journal of Cellular and Molecular Medicine (2016)
Transforming Growth Factor-? and Interleukin-1? Signaling Pathways Converge on the Chemokine CCL20 Promoter*
Oliver J
The Journal of Biological Chemistry (2015)

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