H1038

Hck human

recombinant, expressed in E. coli BL21, vial of ≥200 units

• MDL number: MFCD03792313
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli BL21
• Quality Level: 200
• form: solution
• specific activity: 1-2 unit/μg protein
• packaging: vial of ≥200 units
• relevant disease(s): cancer (leukemia)
• shipped in: dry ice
• storage temp.: −70°C

Description

Application

Hck is a protein-tyrosine kinase that is expressed in hemopoietic cells. It is used in leukemia research .

Biochem/physiol Actions

Hck is a member of the Src tyrosine kinase family. Hck has been shown to associate with and be activated by Bcr-Abl, which is a protein-tyrosine kinase that is expressed in chronic myelogenous leukemia .

Unit Definition

One unit will phosphorylate 1.0 picomole of Tyr¹⁵ of the peptide substrate p^{cdc2 6-20} per minute at 30 °C at pH 7.4.

Physical form

Supplied as a solution in 50 mM Tris HCl, pH 7.4, 1 mM EDTA and 1 mM DTT

Safety Information

• Storage Class Code: 10 - Combustible liquids