GE28-9852-54

rProtein A GraviTrap^™

Cytiva 28-9852-54, pack of 10 × 1 mL

• Synonym(s): GraviTrap
• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• form: prepacked column
• crosslinking: cross-linked
• packaging: pack of 10 × 1 mL
• manufacturer/tradename: Cytiva 28-9852-54
• storage condition: (20% Ethanol)
• technique(s): liquid chromatography (LC): suitable
• bed volume: 1 mL
• matrix: (Highly cross-linked agarose, 4%)
• matrix active group: Protein A functional group
• particle size: (90 µm)
• capacity: 50 mg binding capacity (human IgG/column)(Protein binding capacity is protein-to-protein dependent.)
• separation technique: affinity
• storage temp.: 2-8°C

Description

General description

rProtein A GraviTrap^™ includes ten ready-to-use columns prepacked with 1 mL of rProtein A Sepharose^™ Fast Flow.

Application

rProtein A GraviTrap^™ are prepacked gravity-fLow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on Protein A ligands in gravity-fLow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

Features and Benefits

• Prepacked gravity-fLow columns provide convenience, save time, and increase the reliability of your results.
• Purification of antibodies in 30 min allows you to quickly obtain and store your target Protein in order to avoid the inherent dangers of Protein instability and degradation associated with sLow and long purification runs.
• High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
• High binding capacity such as rProtein A GraviTrap^™ ~ 50 mg human IgG/column

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GraviTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids