GE17-5132-03
Glutathione Sepharose™ 4 Fast Flow
Cytiva 17-5132-03, pack of 500 mL
Synonym(s):
Glutathione Column, Sepharose 4 Fast Flow
About This Item
Recommended Products
ligand
glutathione and 10-carbon linker arm
packaging
pack of 500 mL
manufacturer/tradename
Cytiva 17-5132-03
matrix
highly cross-linked 4% agarose
average diameter
90 μm
cleaning
3-12
working range
3-12
capacity
>10 mg/mL binding capacity
Related Categories
General description
Glutathione Sepharose™ 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.
Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.
Features and Benefits
- Fast, one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- For scale-up due to high flow properties
- Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.
This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.
This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from GE Healthcare.
Protocols
This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.
Characteristics of Glutathione Sepharose® Products
This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.
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