F7250

Fluorescein isothiocyanate isomer I

≥90% purity (HPLC), powder

• Synonym(s): FITC, Fluorescein 5-isothiocyanate
• Empirical Formula (Hill Notation): C₂₁H₁₁NO₅S
• CAS Number: 3326-32-7
• Molecular Weight: 389.38
• Beilstein: 1407295
• EC Number: 222-042-0
• MDL number: MFCD00005063
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894933
• NACRES: NA.47

Properties

• Product Name: Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder
• Quality Level: 200
• Assay: ≥90% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: orange to dark orange
• mp: >360 °C (lit.)
• solubility: acetone: 1 mg/mL
• fluorescence: λ_ex 492 nm; λ_em 518 nm (green)
• suitability: suitable for protein labeling
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description

General description

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.

Application

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Reagent for the FITC labeling of proteins; microsequencing of proteins and peptides (HPLC)
Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves