E3651

Anti-Endothelin Receptor A antibody produced in rabbit

affinity isolated antibody, lyophilized powder

• Synonym(s): Anti-ET-A, Anti-ET1-Specific Endothelin Receptor
• MDL number: MFCD01092540
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: human, pig, chicken, bovine
• technique(s): immunohistochemistry: 1:100 using rat brain sections or rat lung paraffin embedded sections; western blot: 1:200 using rat brain membranes and ECL
• UniProt accession no.: P25101
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EDNRA(1909) ; rat ... Ednra(24326)

Description

Specificity

The epitope is highly conserved in human, swine, bovine and chicken ET-A.

Immunogen

synthetic peptide corresponding to amino acids 413-426 of rat ET-A (with additional N-terminal cysteine).

Application

Anti-Endothelin Receptor A antibody produced in rabbit is suitable for immunohistochemistry at a dilution of 1:100 using rat brain sections or rat lung paraffin embedded sections and western blotting at a dilution of 1:200 using rat brain membranes and ECL.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Biochem/physiol Actions

Endothelin receptor type A (ETA, ETAR) is a human G protein-coupled receptor that mediates vasoconstriction of blood vessels. It is involved in tumor cell invasion, survival and metastasis. ETAR mediates the promotion of ET-1 on colon cancer cell survival, invasion and MMP-2 expression by a PI3K-mediated mechanism. It plays a critical role in colon cancer metastasis.

Physical form

Lyophilized from a solution of phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable