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D1283

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

With calcium chloride and magnesium chloride, 10×, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DPBS

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About This Item

MDL number:
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

components

glucose: no (Dextro)
sodium pyruvate: no

shipped in

ambient

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General description

Dulbecco′s Phosphate Buffered Saline is an isotonic saline solution used for washing cells and tissues. This balanced salt solution (BSS) is also used in cell culture as a buffering system to maintain the medium within the physiological pH range and as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.

Application

Dulbecco′s Phosphate Buffered Saline has been used:
  • for decellularization of extracellular matrix (ECM)
  • to rinse biosensor chip to remove physically adsorbed mycolic acids
  • for washing cells before staining during in vitro biocompatibility test

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Alba Di Pardo et al.
Cell death & disease, 11(7), 546-546 (2020-07-20)
The longevity-associated variant (LAV) of the bactericidal/permeability-increasing fold-containing family B member 4 (BPIFB4) has been found significantly enriched in long-living individuals. Neuroinflammation is a key player in Huntington's disease (HD), a neurodegenerative disorder caused by neural death due to expanded
Melanie A Burkhardt et al.
Biomaterials science, 5(10), 2009-2023 (2017-08-16)
Blood clots stop bleeding and provide cell-instructive microenvironments. Still, in vitro models used to study implant performance typically neglect any possible interactions of recruited cells with surface-adhering blood clots. Here we study the interaction and synergies of bone marrow derived
Janice Dias et al.
Eye & contact lens, 41(5), 281-286 (2015-01-21)
To determine the effect of hydration media on ex vivo corneal elasticity. Experiments were conducted on 40 porcine eyes retrieved from an abattoir (10 eyes each for phosphate-buffered saline (PBS), balanced salt solution, Optisol, 15% dextran). The epithelium was removed
Sarah S Gabriel et al.
Kidney international, 91(4), 842-855 (2017-02-02)
Dent disease is a rare X-linked tubulopathy caused by mutations in the endosomal chloride-proton exchanger (ClC-5) resulting in defective receptor-mediated endocytosis and severe proximal tubule dysfunction. Bone marrow transplantation has recently been shown to preserve kidney function in cystinosis, a
Nasim Shahidi Hamedani et al.
Journal of clinical medicine, 10(4) (2021-02-14)
Coagulation factor XIII (FXIII) is a protransglutaminase which plays an important role in clot stabilization and composition by cross-linking the α- and γ-chains of fibrin and increasing the resistance of the clot to mechanical and proteolytic challenges. In this study

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