111A3

85062504, Application: Fusion partner in the production of heterohybridomas, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Bovine Heterohybridoma
• packaging: tube of 5 μg 85062504-DNA-5UG; pkg of vial of cells 85062504-1VL
• ImmunoDon: Jersey calf lymph node
• Myeloma: 53B3 (mouse x bovine heteromyeloma)
• Reactivity: Application: Fusion partner in the production of heterohybridomas
• technique(s): cell culture | mammalian: suitable
• isotype: Not specified
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse 53B3 x Bovine Jersey Calf lymph node

Cell Line Description

Non Ig secreting

Immunogen

Sheep red blood cells

Application

This line has been used as a HAT sensitive fusion partner in the production of heterohybridomas

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.