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B8400

Sigma-Aldrich

Monoclonal Anti-Bovine IgG2 antibody produced in mouse

clone BG2-7, ascites fluid

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About This Item

MDL number:
MFCD00164545
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

BG2-7, monoclonal

contains

15 mM sodium azide

technique(s)

capture ELISA: suitable
dot blot: suitable
indirect ELISA: 1:1,000
western blot: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

The IgG class antibodies contain two subclasses, IgG1 and IgG2, which have antigenic differences in the Fc portion of the heavy chains. Monoclonal Anti-Bovine IgG2 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes of an immunized mouse.

Immunogen

Purified bovine IgG

Application

Monoclonal Anti-Bovine IgG2 antibody has been used in:
  • western blot
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot assay

Biochem/physiol Actions

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Detection of Bovine IgG Isotypes in a PPA-ELISA for Johne's Disease Diagnosis in Infected Herds
Fernandez B, et al.
Veterinary Medicine International, 2012 (2012)
Measurement of bovine IgG by indirect competitive ELISA as a means of detecting milk adulteration
Hurley IP, et al.
Journal of Dairy Science, 87(3), 543-549 (2004)
Detection of Bovine IgG Isotypes in a PPA-ELISA for Johne's Disease Diagnosis in Infected Herds
Fernandez, B., et al.
Veterinary Medicine International, doi: 10-doi: 10 (2012)
Tom N McNeilly et al.
Vaccine, 28(5), 1412-1421 (2009-11-21)
Systemic immunization of cattle with H7 flagellin results in induction of both H7-specific IgA and IgG antibodies but only partially protects against subsequent colonization with Escherichia coli O157:H7. Recent studies indicate that anti-flagellin antibodies directed against TLR5 binding domains located
I P Hurley et al.
Journal of dairy science, 87(3), 543-549 (2004-06-19)
The aim of this work was to develop an assay capable of detecting adulteration of high premium milk with milk from cheaper sources. An indirect, competitive ELISA was developed for the rapid detection of cows' milk in the milk of

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