B5144

Bromelain from pineapple stem

≥4 units/mg protein, (chromatography purified)

• CAS Number: 37189-34-7
• Enzyme Commission number: 3.4.22.32 (BRENDA, IUBMB)
• EC Number: 253-387-5
• MDL number: MFCD00081423
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: (chromatography purified); ≥4 units/mg protein
• composition: Protein, 30-50% biuret
• solubility: H₂O: soluble 1.0 mg/mL, clear, colorless
• storage temp.: −20°C

Description

Application

Bromelain from pineapple stem has been used as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) zymograms. It has also been used as a Azocasein substrate for the determination of proteolytic activity.

Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .

Biochem/physiol Actions

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.

Unit Definition

One unit will release 1.0 μmole of p-nitrophenol from N-α-Z-L-lysine p-nitrophenyl ester per min at pH 4.6 at 25 °C.

Physical form

Lyophilized powder containing mannitol and potassium phosphate buffer salts

Preparation Note

Chromatographically purified

Chromatographically purified. Dissolves in water at 1 mg/mL concentration to form a clear, colorless solution.

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H315 - H319 - H334 - H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable